Clone: 10123066965770744 D09

Tagged gene: alpha-Est5
Identifier: FBgn0261393

Clone FlyFos017010(pRedFlp-Hgr)(alpha-Est5[22536]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
3350289 3354499 12837 17047 - gene alpha-Est6 coding_transcript
3354586 3355963 17134 18511 - gene Rtnl2 coding_transcript
3356478 3358454 19026 21002 + gene alphaTub84D coding_transcript
3359218 3362247 21766 25857 - gene alpha-Est5 coding_transcript
3359218 3362247 21766 25857 - mrna FBtr0307274 coding_transcript
3359218 3360438 21766 24048 - exon coding_transcript
3359218 3359985 21766 22533 - three_prime_utr coding_transcript
3359951 3362247 22499 25857 - mrna FBtr0081547 coding_transcript
3359951 3360438 22499 24048 - exon coding_transcript
3359951 3359985 22499 22533 - three_prime_utr coding_transcript
3359986 3360438 22534 24048 - cds coding_transcript
3359986 3360438 22534 24048 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 22610 22643 - misc_recomb FRT CLC
2358 2429 22651 22722 - cds BLRP CLC
2337 2357 22723 22743 - cds TEV CLC
2313 2336 22744 22767 - cds Precision cut site CLC
2271 2312 22768 22809 - cds V5 CLC
1548 2264 22816 23532 - cds SGFP CLC
1482 1541 23539 23598 - cds 2xTY1 CLC
dmel-5.43-3R
3360439 3360523 24049 24133 - intron coding_transcript
3360439 3360523 24049 24133 - intron coding_transcript
3360524 3361257 24134 24867 - exon coding_transcript
3360524 3361257 24134 24867 - cds coding_transcript
3360524 3361257 24134 24867 - exon coding_transcript
3360524 3361257 24134 24867 - cds coding_transcript
3361258 3361454 24868 25064 - intron coding_transcript
3361258 3361454 24868 25064 - intron coding_transcript
3361455 3361605 25065 25215 - exon coding_transcript
3361455 3361605 25065 25215 - cds coding_transcript
3361455 3361605 25065 25215 - exon coding_transcript
3361455 3361605 25065 25215 - cds coding_transcript
3361606 3361667 25216 25277 - intron coding_transcript
3361606 3361667 25216 25277 - intron coding_transcript
3361668 3361944 25278 25554 - exon coding_transcript
3361668 3361944 25278 25554 - cds coding_transcript
3361668 3361941 25278 25551 - exon coding_transcript
3361668 3361941 25278 25551 - cds coding_transcript
3361942 3362005 25552 25615 - intron coding_transcript
3361945 3362005 25555 25615 - intron coding_transcript
3362006 3362247 25616 25857 - exon coding_transcript
3362006 3362247 25616 25857 - exon coding_transcript
3362006 3362022 25616 25632 - cds coding_transcript
3362006 3362022 25616 25632 - cds coding_transcript
3362023 3362247 25633 25857 - five_prime_utr coding_transcript
3362023 3362247 25633 25857 - five_prime_utr coding_transcript
3362409 3364333 26019 27943 - gene alpha-Est4 coding_transcript
3364694 3367349 28304 30959 - gene alpha-Est3 coding_transcript
3367533 3370952 31143 34562 + gene alpha-Est2 coding_transcript
3371455 3374844 35065 38454 - gene alpha-Est1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
alpha-Est5[22536]::S000169_fly_pretag_fwd
ATGAAAAACTACAAATTTGGGACAACTTGTATAAGCCAAATCAATTGATCgaagtgcataccaatcaggacccgc
fasta Fasta
alpha-Est5[22536]::S000169_fly_pretag_rev
AAGCAGTACTTTAGTGAACATTAATGCATGGAAATTTATTTGCATGCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta