Clone: 10123066965770744 G01

Tagged gene: ppk10
Identifier: FBgn0065110

Clone FlyFos016945(pRedFlp-Hgr)(ppk10[21260]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
10439440 10440886 10564 12010 + gene CG13142 coding_transcript
10442727 10450080 13851 21204 + gene CG6232 coding_transcript
10450134 10452201 21258 24387 - gene ppk10 coding_transcript
10450134 10452201 21258 24387 - mrna FBtr0100097 coding_transcript
10450134 10450249 21258 22435 - exon coding_transcript
10450134 10450249 21258 22435 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 21334 21367 - misc_recomb FRT CLC
2358 2429 21375 21446 - cds BLRP CLC
2337 2357 21447 21467 - cds TEV CLC
2313 2336 21468 21491 - cds Precision cut site CLC
2271 2312 21492 21533 - cds V5 CLC
1548 2264 21540 22256 - cds SGFP CLC
1482 1541 22263 22322 - cds 2xTY1 CLC
dmel-5.43-2L
10450250 10450305 22436 22491 - intron coding_transcript
10450306 10450420 22492 22606 - exon coding_transcript
10450306 10450420 22492 22606 - cds coding_transcript
10450421 10450490 22607 22676 - intron coding_transcript
10450491 10450597 22677 22783 - exon coding_transcript
10450491 10450597 22677 22783 - cds coding_transcript
10450598 10450676 22784 22862 - intron coding_transcript
10450677 10450739 22863 22925 - exon coding_transcript
10450677 10450739 22863 22925 - cds coding_transcript
10450740 10450794 22926 22980 - intron coding_transcript
10450795 10450949 22981 23135 - exon coding_transcript
10450795 10450949 22981 23135 - cds coding_transcript
10450950 10451011 23136 23197 - intron coding_transcript
10451012 10451130 23198 23316 - exon coding_transcript
10451012 10451130 23198 23316 - cds coding_transcript
10451131 10451194 23317 23380 - intron coding_transcript
10451195 10451395 23381 23581 - exon coding_transcript
10451195 10451395 23381 23581 - cds coding_transcript
10451396 10451451 23582 23637 - intron coding_transcript
10451452 10451704 23638 23890 - exon coding_transcript
10451452 10451704 23638 23890 - cds coding_transcript
10451705 10451760 23891 23946 - intron coding_transcript
10451761 10451852 23947 24038 - exon coding_transcript
10451761 10451852 23947 24038 - cds coding_transcript
10451853 10451906 24039 24092 - intron coding_transcript
10451907 10452012 24093 24198 - exon coding_transcript
10451907 10452012 24093 24198 - cds coding_transcript
10452013 10452070 24199 24256 - intron coding_transcript
10452071 10452201 24257 24387 - exon coding_transcript
10452071 10452201 24257 24387 - cds coding_transcript
10452677 10457241 24863 29427 - gene CG5322 coding_transcript
10457656 10462475 29842 34661 + gene CG6206 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
ppk10[21260]::S000169_fly_pretag_rev
TTTACTTTATTAATGTTGTAAATATTAAACAAAGCATTAGTTTATTCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
ppk10[21260]::S000169_fly_pretag_fwd
TATACTTCTTTACAATTAGAATAGTCTTTCAAATATTGGGTTACACCCTGgaagtgcataccaatcaggacccgc
fasta Fasta