Clone: 10123066965770744 C01

Tagged gene: Dak1
Identifier: FBgn0028833

Clone FlyFos016935(pRedFlp-Hgr)(Dak1[14314]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
21878547 21879850 12562 13865 + gene vps2 coding_transcript
21879959 21881210 13974 16287 - gene Dak1 coding_transcript
21879959 21881210 13974 16287 - mrna FBtr0084976 coding_transcript
21879959 21881210 13974 16287 - exon coding_transcript
21879959 21880296 13974 14311 - three_prime_utr coding_transcript
21880297 21881058 14312 16135 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 14388 14421 - misc_recomb FRT CLC
2358 2429 14429 14500 - cds BLRP CLC
2337 2357 14501 14521 - cds TEV CLC
2313 2336 14522 14545 - cds Precision cut site CLC
2271 2312 14546 14587 - cds V5 CLC
1548 2264 14594 15310 - cds SGFP CLC
1482 1541 15317 15376 - cds 2xTY1 CLC
dmel-5.43-3R
21881059 21881210 16136 16287 - five_prime_utr coding_transcript
21881368 21882213 16445 17290 - gene Sld5 coding_transcript
21882349 21883072 17426 18149 + gene CG14543 coding_transcript
21883069 21883945 18146 19022 - gene CG42498 coding_transcript
21883069 21883945 18146 19022 - gene CG14550 coding_transcript
21884142 21884989 19219 20066 - gene RpL34a coding_transcript
21885218 21887015 20295 22092 + gene CG14544 coding_transcript
21886990 21888733 22067 23810 - gene CG14551 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Dak1[14314]::S000169_fly_pretag_fwd
CGGAAGAGGTGTTCGGGGAGGTGGAAAAGATATTTGTGGCCAGCGGCTTCgaagtgcataccaatcaggacccgc
fasta Fasta
Dak1[14314]::S000169_fly_pretag_rev
AAGGTAAGGATAGCACTACACACATAACCGGAATATGAACAGGAAATCTActtgtcgtcgtcatccttgtagtca
fasta Fasta