Clone: 05512796956240418 B11

Tagged gene: polybromo
Identifier: FBgn0039227

Clone FlyFos016930(pRedFlp-Hgr)(polybromo[36250]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
20610459 20613152 20951 23644 - gene CG5805 coding_transcript
20614072 20615858 24564 26350 - gene CG13634 coding_transcript
20617465 20623678 27957 34170 + gene Ets96B coding_transcript
20623179 20625390 33671 35882 - gene Ude coding_transcript
20625691 20630977 36183 42531 - gene polybromo coding_transcript
20625691 20630977 36183 42531 - mrna FBtr0084740 coding_transcript
20625691 20626723 36183 38277 - exon coding_transcript
20625691 20625755 36183 36247 - three_prime_utr coding_transcript
20625756 20626723 36248 38277 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 36324 36357 - misc_recomb FRT CLC
2358 2429 36365 36436 - cds BLRP CLC
2337 2357 36437 36457 - cds TEV CLC
2313 2336 36458 36481 - cds Precision cut site CLC
2271 2312 36482 36523 - cds V5 CLC
1548 2264 36530 37246 - cds SGFP CLC
1482 1541 37253 37312 - cds 2xTY1 CLC
dmel-5.43-3R
20626724 20626781 38278 38335 - intron coding_transcript
20626782 20628234 38336 39788 - exon coding_transcript
20626782 20628234 38336 39788 - cds coding_transcript
20628235 20628292 39789 39846 - intron coding_transcript
20628293 20629884 39847 41438 - exon coding_transcript
20628293 20629884 39847 41438 - cds coding_transcript
20629885 20629942 41439 41496 - intron coding_transcript
20629943 20630977 41497 42531 - exon coding_transcript
20629943 20630894 41497 42448 - cds coding_transcript
20630895 20630977 42449 42531 - five_prime_utr coding_transcript
20633333 20634403 44887 45957 + gene CG6980 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
polybromo[36250]::S000169_fly_pretag_rev
ATTAACATGGAACTCAAAAAGCTTTGGGCTTAGCGGTGAACGATGGGCTActtgtcgtcgtcatccttgtagtca
fasta Fasta
polybromo[36250]::S000169_fly_pretag_fwd
TGCGCAACTTCATGGTCGACGATATACTTCAAATACGGCGCAGTTGCAACgaagtgcataccaatcaggacccgc
fasta Fasta