Clone: 05512796956240418 G06

Tagged gene: mRpS29
Identifier: FBgn0034727

Clone FlyFos016886(pRedFlp-Hgr)(mRpS29[30575]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
18314984 18317089 20115 22220 - gene ppk12 coding_transcript
18313238 18314470 22734 23966 + gene CG10344 coding_transcript
18308544 18312979 24225 28660 - gene rad50 coding_transcript
18306442 18307882 29322 31824 + gene mRpS29 coding_transcript
18306442 18307882 29322 31824 + mrna FBtr0071842 coding_transcript
18306442 18307882 29322 31824 + exon coding_transcript
18307805 18307882 29322 29399 + five_prime_utr coding_transcript
18306626 18307804 29400 31640 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30576 30635 + cds 2xTY1 CLC
1548 2264 30642 31358 + cds SGFP CLC
2271 2312 31365 31406 + cds V5 CLC
2313 2336 31407 31430 + cds Precision cut site CLC
2337 2357 31431 31451 + cds TEV CLC
2358 2429 31452 31523 + cds BLRP CLC
3813 3846 31531 31564 + misc_recomb FRT CLC
dmel-5.43-2R
18306442 18306625 31641 31824 + three_prime_utr coding_transcript
18303318 18306209 32057 34948 - gene qkr58E-2 coding_transcript
18300422 18303047 35219 37844 + gene qkr58E-1 coding_transcript
18299312 18300087 38179 38954 - gene Mes4 coding_transcript
18296797 18299049 39217 41469 + gene qkr58E-3 coding_transcript
18296155 18296847 41419 42111 - gene ventrally-expressed-protein-D coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
mRpS29[30575]::S000169_fly_pretag_rev
ACAAATCATTTAACTTTATTGGCTAACAAATACAGGACACTCGCTCTCTActtgtcgtcgtcatccttgtagtca
fasta Fasta
mRpS29[30575]::S000169_fly_pretag_fwd
TCATGAGCAACAAGAACCCCTACTCGCTGATGCAGCTCGTGAAGGCCTTGgaagtgcataccaatcaggacccgc
fasta Fasta