Clone: 05512796956240418 A02

Tagged gene: Hsp27
Identifier: FBgn0001226

Clone FlyFos016823(pRedFlp-Hgr)(Hsp27[27110]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
9360484 9363400 9670 12586 + gene CG4452 coding_transcript
9363295 9364338 12481 13524 - gene Fdxh coding_transcript
9364611 9365583 13797 14769 - gene Hsp67Bc coding_transcript
9365822 9368064 15008 17250 + gene Hsp67Bb coding_transcript
9366031 9368064 15217 17250 + gene Hsp22 coding_transcript
9368530 9369393 17716 18579 + gene CG4461 coding_transcript
9369518 9370527 18704 19713 - gene Hsp26 coding_transcript
9370902 9372634 20088 21820 - gene Hsp67Ba coding_transcript
9374986 9375865 24172 25051 + gene Hsp23 coding_transcript
9377165 9378382 26351 28630 + gene Hsp27 coding_transcript
9377165 9378382 26351 28630 + mrna FBtr0076454 coding_transcript
9377165 9378382 26351 28630 + exon coding_transcript
9377165 9377285 26351 26471 + five_prime_utr coding_transcript
9377286 9377927 26472 28175 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 27111 27170 + cds 2xTY1 CLC
1548 2264 27177 27893 + cds SGFP CLC
2271 2312 27900 27941 + cds V5 CLC
2313 2336 27942 27965 + cds Precision cut site CLC
2337 2357 27966 27986 + cds TEV CLC
2358 2429 27987 28058 + cds BLRP CLC
3813 3846 28066 28099 + misc_recomb FRT CLC
dmel-5.43-3L
9377928 9378382 28176 28630 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Hsp27[27110]::S000169_fly_pretag_fwd
GCGATGGAAAAGCCGAAAATGGCAGCGGCGAGAAAATGGAGACTAGCAAGgaagtgcataccaatcaggacccgc
fasta Fasta
Hsp27[27110]::S000169_fly_pretag_rev
TTCCTCTTCTCGTCTTCTTCATCTCCTAGTCTTCCTCTTTTCGTCTTTTActtgtcgtcgtcatccttgtagtca
fasta Fasta