Clone: 3207692103829951 B03

Tagged gene: Npc2d
Identifier: FBgn0037782

Clone FlyFos016686(pRedFlp-Hgr)(Npc2d[26361]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
5893627 5894294 13283 13950 - gene CG33784 coding_transcript
5892492 5893289 14288 15085 - gene CG33783 coding_transcript
5889729 5890367 17210 17848 - gene CG33631 coding_transcript
5888346 5889049 18528 19231 - gene CG33630 coding_transcript
5886019 5886858 20719 21558 - gene Npc2c coding_transcript
5881078 5881805 25772 27561 + gene Npc2d coding_transcript
5881078 5881805 25772 27561 + mrna FBtr0082202 coding_transcript
5881708 5881805 25772 25869 + exon coding_transcript
5881802 5881805 25772 25775 + five_prime_utr coding_transcript
5881708 5881801 25776 25869 + cds coding_transcript
5881641 5881707 25870 25936 + intron coding_transcript
5881078 5881640 25937 27561 + exon coding_transcript
5881213 5881640 25937 27426 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 26362 26421 + cds 2xTY1 CLC
1548 2264 26428 27144 + cds SGFP CLC
2271 2312 27151 27192 + cds V5 CLC
2313 2336 27193 27216 + cds Precision cut site CLC
2337 2357 27217 27237 + cds TEV CLC
2358 2429 27238 27309 + cds BLRP CLC
3813 3846 27317 27350 + misc_recomb FRT CLC
dmel-5.43-3R
5881078 5881212 27427 27561 + three_prime_utr coding_transcript
5879120 5879982 28657 29519 - gene Npc2e coding_transcript
5877230 5878585 30054 31409 + gene Fancl coding_transcript
5874889 5877249 31390 33750 - gene CG3925 coding_transcript
5872974 5874289 34350 35665 + gene CG12811 coding_transcript
5871298 5872982 35657 37341 - gene mtTFB2 coding_transcript
5870318 5871245 37394 38321 + gene CG11722 coding_transcript
5868980 5870328 38311 39659 - gene CG3909 coding_transcript

Sets

Oligos

Name/Sequence Export
Npc2d[26361]::S000169_fly_pretag_fwd
TGGTCAAGGGCAACGGCGGCAATACGGTCTATGAGCTGGACTATCTCAACgaagtgcataccaatcaggacccgc
 fasta Fasta
Npc2d[26361]::S000169_fly_pretag_rev
TATATCACAGTAATTTAAACCAATTGATGAATTAACTAGGCCCAACTTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta