Clone: 7757956326115614 B12

Tagged gene: CG31954
Identifier: FBgn0051954

Clone FlyFos016657(pRedFlp-Hgr)(CG31954[37254]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
3530903 3532526 13903 15526 - gene CG8853 coding_transcript
3527387 3530317 16112 19042 + gene Spindly coding_transcript
3525133 3527588 18841 21296 - gene CG8852 coding_transcript
3522594 3523960 22469 23835 + gene Sr-CIV coding_transcript
3520262 3522555 23874 26167 - gene CG8851 coding_transcript
3517273 3519697 26732 29156 + gene CG3213 coding_transcript
3514737 3517111 29318 31692 - gene CG17593 coding_transcript
3510477 3514662 31767 35952 + gene CG33123 coding_transcript
3509058 3510082 36347 38433 + gene CG31954 coding_transcript
3509058 3510082 36347 38433 + mrna FBtr0077563 coding_transcript
3509795 3510082 36347 36634 + exon coding_transcript
3510073 3510082 36347 36356 + five_prime_utr coding_transcript
3509795 3510072 36357 36634 + cds coding_transcript
3509728 3509794 36635 36701 + intron coding_transcript
3509058 3509727 36702 38433 + exon coding_transcript
3509172 3509727 36702 38319 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 37255 37314 + cds 2xTY1 CLC
1548 2264 37321 38037 + cds SGFP CLC
2271 2312 38044 38085 + cds V5 CLC
2313 2336 38086 38109 + cds Precision cut site CLC
2337 2357 38110 38130 + cds TEV CLC
2358 2429 38131 38202 + cds BLRP CLC
3813 3846 38210 38243 + misc_recomb FRT CLC
dmel-5.43-2L
3509058 3509171 38320 38433 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG31954[37254]::S000169_fly_pretag_fwd
ACTCAAGAGTAAGTTTCGCACGGGATTGGATCAAGGAGCACAGTGGTGTCgaagtgcataccaatcaggacccgc
fasta Fasta
CG31954[37254]::S000169_fly_pretag_rev
GGAAGAAATTGATTTCAAACTATGTTAACTGTGCAGGATATACGAATCTActtgtcgtcgtcatccttgtagtca
fasta Fasta