Clone: 7757956326115614 A11

Tagged gene: 14-3-3epsilon
Identifier: FBgn0020238

Clone FlyFos016634(pRedFlp-Hgr)(14-3-3epsilon[34097]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
14050624 14051769 10714 11859 - gene CG7168 coding_transcript
14052113 14054440 12203 14530 + gene Mdh2 coding_transcript
14061916 14065324 22006 25414 + gene repo coding_transcript
14065142 14067906 25232 27996 - gene CG7156 coding_transcript
14068253 14075030 28343 36182 + gene 14-3-3epsilon coding_transcript
14068253 14075030 28343 36182 + mrna FBtr0083566 coding_transcript
14068253 14075030 28343 36182 + mrna FBtr0083568 coding_transcript
14068253 14068631 28343 28721 + exon coding_transcript
14068253 14068631 28343 28721 + exon coding_transcript
14068253 14068567 28343 28657 + five_prime_utr coding_transcript
14068253 14068567 28343 28657 + five_prime_utr coding_transcript
14068409 14074301 28499 35453 + mrna FBtr0083565 coding_transcript
14068409 14074301 28499 35453 + mrna FBtr0083567 coding_transcript
14068409 14068631 28499 28721 + exon coding_transcript
14068409 14068631 28499 28721 + exon coding_transcript
14068409 14068567 28499 28657 + five_prime_utr coding_transcript
14068409 14068567 28499 28657 + five_prime_utr coding_transcript
14068568 14068631 28658 28721 + cds coding_transcript
14068568 14068631 28658 28721 + cds coding_transcript
14068568 14068631 28658 28721 + cds coding_transcript
14068568 14068631 28658 28721 + cds coding_transcript
14068632 14073154 28722 33244 + intron coding_transcript
14068632 14073154 28722 33244 + intron coding_transcript
14068632 14073154 28722 33244 + intron coding_transcript
14068632 14073154 28722 33244 + intron coding_transcript
14073155 14073624 33245 33714 + exon coding_transcript
14073155 14073624 33245 33714 + cds coding_transcript
14073155 14073624 33245 33714 + exon coding_transcript
14073155 14073624 33245 33714 + cds coding_transcript
14073155 14073624 33245 33714 + exon coding_transcript
14073155 14073624 33245 33714 + cds coding_transcript
14073155 14073624 33245 33714 + exon coding_transcript
14073155 14073624 33245 33714 + cds coding_transcript
14073625 14073692 33715 33782 + intron coding_transcript
14073625 14073692 33715 33782 + intron coding_transcript
14073625 14073692 33715 33782 + intron coding_transcript
14073625 14073692 33715 33782 + intron coding_transcript
14073693 14073873 33783 33963 + exon coding_transcript
14073693 14073873 33783 33963 + cds coding_transcript
14073693 14073873 33783 33963 + exon coding_transcript
14073693 14073873 33783 33963 + cds coding_transcript
14073693 14073873 33783 33963 + exon coding_transcript
14073693 14073873 33783 33963 + cds coding_transcript
14073693 14073873 33783 33963 + exon coding_transcript
14073693 14073873 33783 33963 + cds coding_transcript
14073874 14073954 33964 34044 + intron coding_transcript
14073874 14073942 33964 34032 + intron coding_transcript
14073874 14073939 33964 34029 + intron coding_transcript
14073874 14073936 33964 34026 + intron coding_transcript
14073937 14074301 34027 35453 + exon coding_transcript
14073937 14074010 34027 35162 + cds coding_transcript
14073940 14075030 34030 36182 + exon coding_transcript
14073940 14074010 34030 35162 + cds coding_transcript
14073943 14075030 34033 36182 + exon coding_transcript
14073943 14074010 34033 35162 + cds coding_transcript
14073955 14074301 34045 35453 + exon coding_transcript
14073955 14074010 34045 35162 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 34098 34157 + cds 2xTY1 CLC
1548 2264 34164 34880 + cds SGFP CLC
2271 2312 34887 34928 + cds V5 CLC
2313 2336 34929 34952 + cds Precision cut site CLC
2337 2357 34953 34973 + cds TEV CLC
2358 2429 34974 35045 + cds BLRP CLC
3813 3846 35053 35086 + misc_recomb FRT CLC
dmel-5.43-3R
14074011 14075030 35163 36182 + three_prime_utr coding_transcript
14074011 14075030 35163 36182 + three_prime_utr coding_transcript
14074011 14074301 35163 35453 + three_prime_utr coding_transcript
14074011 14074301 35163 35453 + three_prime_utr coding_transcript
14076836 14077261 37988 38413 + gene CG18598 coding_transcript
14077220 14078450 38372 39602 - gene CG12320 coding_transcript
14078565 14081191 39717 42343 - gene Rpb4 coding_transcript
14078565 14081191 39717 42343 - gene Ada2a coding_transcript
14081252 14083399 42404 44551 + gene moi coding_transcript
14081252 14083399 42404 44551 + gene tatl coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
14-3-3epsilon[34097]::S000169_fly_pretag_rev
GAAGGTATATAATGTGTTTTAAATGGAAGGGCGGGGGTTTACTTAAATTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
14-3-3epsilon[34097]::S000169_fly_pretag_fwd
ATGGCGAGCCCAAAGAGCAAATCCAGGATGTTGAGGATCAGGACGTGTCGgaagtgcataccaatcaggacccgc
 fasta Fasta