Clone: 02182476214419926 H11

Tagged gene: Fie
Identifier: FBgn0026592

Clone FlyFos015888(pRedFlp-Hgr)(Fie[19671]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
3899259 3903138 10789 14668 + gene Ubi-p63E coding_transcript
3897444 3899156 14771 16483 + gene CG14980 coding_transcript
3896900 3897498 16429 17027 - gene CG14977 coding_transcript
3894999 3896603 17324 18928 + gene Gr63a coding_transcript
3892574 3894833 19094 22415 - gene Fie coding_transcript
3892574 3894833 19094 22415 - mrna FBtr0073157 coding_transcript
3894169 3894833 19094 20820 - exon coding_transcript
3894259 3894833 19094 19668 - three_prime_utr coding_transcript
3894169 3894258 19669 20820 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19745 19778 - misc_recomb FRT CLC
2358 2429 19786 19857 - cds BLRP CLC
2337 2357 19858 19878 - cds TEV CLC
2313 2336 19879 19902 - cds Precision cut site CLC
2271 2312 19903 19944 - cds V5 CLC
1548 2264 19951 20667 - cds SGFP CLC
1482 1541 20674 20733 - cds 2xTY1 CLC
dmel-5.43-3L
3894115 3894168 20821 20874 - intron coding_transcript
3893978 3894114 20875 21011 - exon coding_transcript
3893978 3894114 20875 21011 - cds coding_transcript
3893915 3893977 21012 21074 - intron coding_transcript
3893533 3893914 21075 21456 - exon coding_transcript
3893533 3893914 21075 21456 - cds coding_transcript
3892990 3893532 21457 21999 - intron coding_transcript
3892574 3892989 22000 22415 - exon coding_transcript
3892846 3892989 22000 22143 - cds coding_transcript
3892574 3892845 22144 22415 - five_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Fie[19671]::S000169_fly_pretag_rev
GACACCTGATGGGAAGTTCCAAAAATATCCAATCTTTCAGTACATGTTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Fie[19671]::S000169_fly_pretag_fwd
GCAGCAATGGATATAAGCACATTCTAACCGAGTCTCCCGATGGACAGTGTgaagtgcataccaatcaggacccgc
fasta Fasta