Clone: 02182476214419926 G09
Tagged gene: CG30049
Identifier: FBgn0050049
| Clone | FlyFos015851(pRedFlp-Hgr)(CG30049[12848]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
Validation
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | good | [view] |
Genes in pFlyFos vector
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-2R | ||||||||
| 8243176 | 8246592 | 12731 | 17209 | - | gene | CG30049 | coding_transcript | |
| 8243176 | 8246592 | 12731 | 17209 | - | mrna | FBtr0087906 | coding_transcript | |
| 8246310 | 8246592 | 12731 | 14075 | - | exon | coding_transcript | ||
| 8246478 | 8246592 | 12731 | 12845 | - | three_prime_utr | coding_transcript | ||
| 8246310 | 8246477 | 12846 | 14075 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 12922 | 12955 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 12963 | 13034 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 13035 | 13055 | - | cds | TEV | CLC | |
| 2313 | 2336 | 13056 | 13079 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 13080 | 13121 | - | cds | V5 | CLC | |
| 1548 | 2264 | 13128 | 13844 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 13851 | 13910 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-2R | ||||||||
| 8246254 | 8246309 | 14076 | 14131 | - | intron | coding_transcript | ||
| 8245753 | 8246253 | 14132 | 14632 | - | exon | coding_transcript | ||
| 8245753 | 8246253 | 14132 | 14632 | - | cds | coding_transcript | ||
| 8245700 | 8245752 | 14633 | 14685 | - | intron | coding_transcript | ||
| 8245556 | 8245699 | 14686 | 14829 | - | exon | coding_transcript | ||
| 8245556 | 8245699 | 14686 | 14829 | - | cds | coding_transcript | ||
| 8245504 | 8245555 | 14830 | 14881 | - | intron | coding_transcript | ||
| 8245319 | 8245503 | 14882 | 15066 | - | exon | coding_transcript | ||
| 8245319 | 8245503 | 14882 | 15066 | - | cds | coding_transcript | ||
| 8245252 | 8245318 | 15067 | 15133 | - | intron | coding_transcript | ||
| 8245071 | 8245251 | 15134 | 15314 | - | exon | coding_transcript | ||
| 8245071 | 8245251 | 15134 | 15314 | - | cds | coding_transcript | ||
| 8245010 | 8245070 | 15315 | 15375 | - | intron | coding_transcript | ||
| 8244626 | 8245009 | 15376 | 15759 | - | exon | coding_transcript | ||
| 8244626 | 8245009 | 15376 | 15759 | - | cds | coding_transcript | ||
| 8244569 | 8244625 | 15760 | 15816 | - | intron | coding_transcript | ||
| 8244414 | 8244568 | 15817 | 15971 | - | exon | coding_transcript | ||
| 8244414 | 8244568 | 15817 | 15971 | - | cds | coding_transcript | ||
| 8244359 | 8244413 | 15972 | 16026 | - | intron | coding_transcript | ||
| 8244238 | 8244358 | 16027 | 16147 | - | exon | coding_transcript | ||
| 8244238 | 8244358 | 16027 | 16147 | - | cds | coding_transcript | ||
| 8244184 | 8244237 | 16148 | 16201 | - | intron | coding_transcript | ||
| 8244099 | 8244183 | 16202 | 16286 | - | exon | coding_transcript | ||
| 8244099 | 8244183 | 16202 | 16286 | - | cds | coding_transcript | ||
| 8244050 | 8244098 | 16287 | 16335 | - | intron | coding_transcript | ||
| 8243875 | 8244049 | 16336 | 16510 | - | exon | coding_transcript | ||
| 8243875 | 8244049 | 16336 | 16510 | - | cds | coding_transcript | ||
| 8243817 | 8243874 | 16511 | 16568 | - | intron | coding_transcript | ||
| 8243306 | 8243816 | 16569 | 17079 | - | exon | coding_transcript | ||
| 8243306 | 8243816 | 16569 | 17079 | - | cds | coding_transcript | ||
| 8243247 | 8243305 | 17080 | 17138 | - | intron | coding_transcript | ||
| 8243176 | 8243246 | 17139 | 17209 | - | exon | coding_transcript | ||
| 8243220 | 8243246 | 17139 | 17165 | - | cds | coding_transcript | ||
| 8243176 | 8243219 | 17166 | 17209 | - | five_prime_utr | coding_transcript | ||
| 8239298 | 8242918 | 17467 | 21087 | - | gene | CG30047 | coding_transcript | |
| 8232591 | 8238583 | 21802 | 27794 | - | gene | ana3 | coding_transcript | |
| 8227115 | 8232118 | 28267 | 33270 | + | gene | CG8839 | coding_transcript | |
| 8225307 | 8227050 | 33335 | 35078 | - | gene | Den1 | coding_transcript | |
| 8224487 | 8225223 | 35162 | 35898 | - | gene | CG34021 | coding_transcript | |
| 8222822 | 8223710 | 36675 | 37563 | - | gene | CG8490 | coding_transcript | |
| 8214179 | 8221324 | 39061 | 46206 | - | gene | garz | coding_transcript | |
Sets
Oligos
| Name/Sequence | Export |
|---|---|
|
CG30049[12848]::S000169_fly_pretag_fwd
ACTTTGTTTATACCATGGAATGGCCATCTCTGTTTATGCGTTACATCTTCgaagtgcataccaatcaggacccgc
|
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|
CG30049[12848]::S000169_fly_pretag_rev
CCGCGAATATAATGGATGCAAACTGAATGATCCACAGGAAGTGGTTCCTActtgtcgtcgtcatccttgtagtca
|
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