Clone: 02182476214419926 H07

Tagged gene: mRpL33
Identifier: FBgn0040907

Clone FlyFos015835(pRedFlp-Hgr)(mRpL33[35842]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
4194185 4196379 14174 16368 - gene CG12692 coding_transcript
4205397 4205832 25386 25821 - gene CG15579 coding_transcript
4208263 4212834 28252 32823 - gene mei-9 coding_transcript
4213473 4215778 33462 35767 + gene CG2982 coding_transcript
4215797 4216234 35786 37285 - gene mRpL33 coding_transcript
4215797 4216234 35786 37285 - mrna FBtr0070655 coding_transcript
4215797 4215900 35786 36951 - exon coding_transcript
4215797 4215850 35786 35839 - three_prime_utr coding_transcript
4215851 4215900 35840 36951 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 35916 35949 - misc_recomb FRT CLC
2358 2429 35957 36028 - cds BLRP CLC
2337 2357 36029 36049 - cds TEV CLC
2313 2336 36050 36073 - cds Precision cut site CLC
2271 2312 36074 36115 - cds V5 CLC
1548 2264 36122 36838 - cds SGFP CLC
1482 1541 36845 36904 - cds 2xTY1 CLC
dmel-5.43-X
4215901 4215963 36952 37014 - intron coding_transcript
4215964 4216067 37015 37118 - exon coding_transcript
4215964 4216067 37015 37118 - cds coding_transcript
4216068 4216123 37119 37174 - intron coding_transcript
4216124 4216234 37175 37285 - exon coding_transcript
4216124 4216164 37175 37215 - cds coding_transcript
4216165 4216234 37216 37285 - five_prime_utr coding_transcript
4221113 4223336 42164 44387 - gene CG12693 coding_transcript

Sets

Oligos

Name/Sequence Export
mRpL33[35842]::S000169_fly_pretag_rev
CCAATAGTTGTAAATTTTATTTTTTTCACTAACAAAAAATGGTTATCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
mRpL33[35842]::S000169_fly_pretag_fwd
CAGTTCAACAGGAAAGCCTTTATCGTGAACGCAAGAAAATCCGCAGCGCCgaagtgcataccaatcaggacccgc
fasta Fasta