Clone: 02182476214419926 A05

Tagged gene: Pxt
Identifier: FBgn0261987

Clone FlyFos015812(pRedFlp-Hgr)(Pxt[25407]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
13545174 13548677 22076 26641 + gene Pxt coding_transcript
13545174 13548677 22076 26641 + mrna FBtr0083508 coding_transcript
13545174 13545458 22076 22360 + exon coding_transcript
13545174 13545292 22076 22194 + five_prime_utr coding_transcript
13545293 13545458 22195 22360 + cds coding_transcript
13545459 13545752 22361 22654 + intron coding_transcript
13545753 13546540 22655 23442 + exon coding_transcript
13545753 13546540 22655 23442 + cds coding_transcript
13546541 13546672 23443 23574 + intron coding_transcript
13546673 13547099 23575 24001 + exon coding_transcript
13546673 13547099 23575 24001 + cds coding_transcript
13547100 13547227 24002 24129 + intron coding_transcript
13547228 13547555 24130 24457 + exon coding_transcript
13547228 13547555 24130 24457 + cds coding_transcript
13547556 13547616 24458 24518 + intron coding_transcript
13547617 13547791 24519 24693 + exon coding_transcript
13547617 13547791 24519 24693 + cds coding_transcript
13547792 13547853 24694 24755 + intron coding_transcript
13547854 13548012 24756 24914 + exon coding_transcript
13547854 13548012 24756 24914 + cds coding_transcript
13548013 13548066 24915 24968 + intron coding_transcript
13548067 13548386 24969 25288 + exon coding_transcript
13548067 13548386 24969 25288 + cds coding_transcript
13548387 13548441 25289 25343 + intron coding_transcript
13548442 13548677 25344 26641 + exon coding_transcript
13548442 13548508 25344 26472 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 25408 25467 + cds 2xTY1 CLC
1548 2264 25474 26190 + cds SGFP CLC
2271 2312 26197 26238 + cds V5 CLC
2313 2336 26239 26262 + cds Precision cut site CLC
2337 2357 26263 26283 + cds TEV CLC
2358 2429 26284 26355 + cds BLRP CLC
3813 3846 26363 26396 + misc_recomb FRT CLC
dmel-5.43-3R
13548509 13548677 26473 26641 + three_prime_utr coding_transcript
13550146 13550766 28110 28730 - gene Atg8b coding_transcript
13551248 13558952 29212 36916 + gene Fsh coding_transcript
13559322 13560103 37286 38067 + gene CG14321 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Pxt[25407]::S000169_fly_pretag_fwd
GCTGTGATGATCCCAACCTGCCTTCTGTTAACTTGGAGGCATGGCGTGCTgaagtgcataccaatcaggacccgc
fasta Fasta
Pxt[25407]::S000169_fly_pretag_rev
TTTAAAATATCCATAAATAAATTAAAAGTGGATGCAAAAAAAGTGCCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta