Clone: 02182476214419926 D03

Tagged gene: grk
Identifier: FBgn0001137

Clone FlyFos015790(pRedFlp-Hgr)(grk[41189]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation bad
Whole tag sequencing full download [show/hide details]

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
8445861 8449571 23163 26873 + gene U26 coding_transcript
8442447 8445660 27074 30287 - gene CG31897 coding_transcript
8437411 8442352 30382 35323 - gene mus201 coding_transcript
8437411 8442352 30382 35323 - gene Chrac-14 coding_transcript
8434058 8437346 35388 38676 + gene D12 coding_transcript
8431086 8433598 39136 42710 + gene grk coding_transcript
8431086 8433598 39136 42710 + mrna FBtr0079708 coding_transcript
8433415 8433598 39136 39319 + exon coding_transcript
8433415 8433598 39136 39319 + five_prime_utr coding_transcript
8433273 8433414 39320 39461 + intron coding_transcript
8433013 8433272 39462 39721 + exon coding_transcript
8433083 8433272 39462 39651 + five_prime_utr coding_transcript
8433013 8433082 39652 39721 + cds coding_transcript
8432495 8433012 39722 40239 + intron coding_transcript
8431877 8432494 40240 40857 + exon coding_transcript
8431877 8432494 40240 40857 + cds coding_transcript
8431742 8431876 40858 40992 + intron coding_transcript
8431086 8431741 40993 42710 + exon coding_transcript
8431542 8431741 40993 42254 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 41190 41249 + cds 2xTY1 CLC
1548 2264 41256 41972 + cds SGFP CLC
2271 2312 41979 42020 + cds V5 CLC
2313 2336 42021 42044 + cds Precision cut site CLC
2337 2357 42045 42065 + cds TEV CLC
2358 2429 42066 42137 + cds BLRP CLC
3813 3846 42145 42178 + misc_recomb FRT CLC
dmel-5.43-2L
8431086 8431541 42255 42710 + three_prime_utr coding_transcript

Sets

Oligos

Name/Sequence Export
grk[41189]::S000169_fly_pretag_fwd
GGAAGCAGCAGCAACTACACCTGCATAAGCAGCGCTTTTTTGTCAGATGCgaagtgcataccaatcaggacccgc
 fasta Fasta
grk[41189]::S000169_fly_pretag_rev
GGCTTATATAGGTGAAACCAAATTGTTTCCAAATCAAATTCTAAATCTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta