Clone: 02182476214419926 B01

Tagged gene: membrin
Identifier: FBgn0260856

Clone FlyFos015765(pRedFlp-Hgr)(membrin[18757]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
5360441 5361108 18065 18732 + gene CG10635 coding_transcript
5359585 5360481 18692 20650 - gene membrin coding_transcript
5359585 5360481 18692 20650 - mrna FBtr0077145 coding_transcript
5359905 5360481 18692 20330 - exon coding_transcript
5360419 5360481 18692 18754 - three_prime_utr coding_transcript
5359905 5360418 18755 20330 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 18831 18864 - misc_recomb FRT CLC
2358 2429 18872 18943 - cds BLRP CLC
2337 2357 18944 18964 - cds TEV CLC
2313 2336 18965 18988 - cds Precision cut site CLC
2271 2312 18989 19030 - cds V5 CLC
1548 2264 19037 19753 - cds SGFP CLC
1482 1541 19760 19819 - cds 2xTY1 CLC
dmel-5.43-3L
5359836 5359904 20331 20399 - intron coding_transcript
5359585 5359835 20400 20650 - exon coding_transcript
5359699 5359835 20400 20536 - cds coding_transcript
5359585 5359698 20537 20650 - five_prime_utr coding_transcript
5355984 5359325 20910 24251 + gene Uev1A coding_transcript
5353290 5355898 24337 26945 - gene CG4769 coding_transcript
5350321 5352354 27881 29914 + gene Klp64D coding_transcript
5336715 5349023 31212 43520 + gene lama coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
membrin[18757]::S000169_fly_pretag_fwd
TGGTGGTCACCTTGCTTATCATCGCCCTGATCATCTATTTCCTAGTGCTCgaagtgcataccaatcaggacccgc
fasta Fasta
membrin[18757]::S000169_fly_pretag_rev
AATAGAGTACATTTAATTTAAACTCAACTAAACGCGATGTCTTCAGATTActtgtcgtcgtcatccttgtagtca
fasta Fasta