Clone: 14763769501329638 E10

Tagged gene: rtet
Identifier: FBgn0028468

Clone FlyFos015736(pRedFlp-Hgr)(rtet[38118]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
16908758 16923527 10450 25219 - gene RhoGAP93B coding_transcript
16923892 16927436 25584 29128 - gene CG7044 coding_transcript
16927936 16930835 29628 32527 + gene CG5745 coding_transcript
16930943 16933686 32635 35378 - gene sec15 coding_transcript
16934170 16936554 35862 39308 + gene rtet coding_transcript
16934170 16936554 35862 39308 + mrna FBtr0084009 coding_transcript
16934170 16934772 35862 36464 + exon coding_transcript
16934170 16934328 35862 36020 + five_prime_utr coding_transcript
16934329 16934772 36021 36464 + cds coding_transcript
16934773 16934968 36465 36660 + intron coding_transcript
16934969 16935310 36661 37002 + exon coding_transcript
16934969 16935310 36661 37002 + cds coding_transcript
16935311 16935373 37003 37065 + intron coding_transcript
16935374 16935447 37066 37139 + exon coding_transcript
16935374 16935447 37066 37139 + cds coding_transcript
16935448 16935740 37140 37432 + intron coding_transcript
16935741 16935916 37433 37608 + exon coding_transcript
16935741 16935916 37433 37608 + cds coding_transcript
16935917 16935973 37609 37665 + intron coding_transcript
16935974 16936279 37666 37971 + exon coding_transcript
16935974 16936279 37666 37971 + cds coding_transcript
16936280 16936337 37972 38029 + intron coding_transcript
16936338 16936554 38030 39308 + exon coding_transcript
16936338 16936429 38030 39183 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 38119 38178 + cds 2xTY1 CLC
1548 2264 38185 38901 + cds SGFP CLC
2271 2312 38908 38949 + cds V5 CLC
2313 2336 38950 38973 + cds Precision cut site CLC
2337 2357 38974 38994 + cds TEV CLC
2358 2429 38995 39066 + cds BLRP CLC
3813 3846 39074 39107 + misc_recomb FRT CLC
dmel-5.43-3R
16936430 16936554 39184 39308 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
rtet[38118]::S000169_fly_pretag_fwd
GCGGACTTCTGCTCATTTATCCAGCCATGGCCTTGCAACGCGCTCGCATTgaagtgcataccaatcaggacccgc
fasta Fasta
rtet[38118]::S000169_fly_pretag_rev
ACTTCCGTTGATCTCTAGTGAGTTTAAAAACTCTATTCCTATTGCACTTActtgtcgtcgtcatccttgtagtca
fasta Fasta