Clone: 14763769501329638 E08

Tagged gene: CkIIalpha-i1
Identifier: FBgn0015025

Clone FlyFos015722(pRedFlp-Hgr)(CkIIalpha-i1[28893]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
15828855 15830580 9749 11474 + gene CG15715 coding_transcript
15826895 15828486 11843 13434 + gene CG18081 coding_transcript
15825560 15827043 13286 14769 - gene CG12713 coding_transcript
15819980 15825104 15225 20349 - gene dbo coding_transcript
15811834 15819520 20809 28495 + gene Dcp2 coding_transcript
15810090 15811721 28608 31301 - gene CkIIalpha-i1 coding_transcript
15810090 15811721 28608 31301 - mrna FBtr0075504 coding_transcript
15810332 15811721 28608 31059 - exon coding_transcript
15811439 15811721 28608 28890 - three_prime_utr coding_transcript
15810332 15811438 28891 31059 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 28967 29000 - misc_recomb FRT CLC
2358 2429 29008 29079 - cds BLRP CLC
2337 2357 29080 29100 - cds TEV CLC
2313 2336 29101 29124 - cds Precision cut site CLC
2271 2312 29125 29166 - cds V5 CLC
1548 2264 29173 29889 - cds SGFP CLC
1482 1541 29896 29955 - cds 2xTY1 CLC
dmel-5.43-3L
15810274 15810331 31060 31117 - intron coding_transcript
15810090 15810273 31118 31301 - exon coding_transcript
15810148 15810273 31118 31243 - cds coding_transcript
15810090 15810147 31244 31301 - five_prime_utr coding_transcript
15803218 15809515 31876 38173 + gene fwe coding_transcript
15802598 15802983 38408 38793 - gene CG13445 coding_transcript
15801518 15801819 39572 39873 - gene CG6244 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CkIIalpha-i1[28893]::S000169_fly_pretag_fwd
TTTCCCTGCAGAAGGGAAAATACATCAATCTTTCGGACAATTCAAGACCTgaagtgcataccaatcaggacccgc
fasta Fasta
CkIIalpha-i1[28893]::S000169_fly_pretag_rev
TTTAATCAGTTTTCAAGATTACCATTATTTGCTAGCCTAGTTGGTAACTActtgtcgtcgtcatccttgtagtca
fasta Fasta