Clone: 14763769501329638 A08

Tagged gene: mid
Identifier: FBgn0261963

Clone FlyFos015718(pRedFlp-Hgr)(mid[43157]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
5461641 5467610 38089 45120 + gene mid coding_transcript
5461641 5467610 38089 45120 + mrna FBtr0079093 coding_transcript
5461641 5466829 38089 44339 + mrna FBtr0310358 coding_transcript
5461641 5462104 38089 38552 + exon coding_transcript
5461641 5462104 38089 38552 + exon coding_transcript
5461641 5461917 38089 38365 + five_prime_utr coding_transcript
5461641 5461917 38089 38365 + five_prime_utr coding_transcript
5461918 5462104 38366 38552 + cds coding_transcript
5461918 5462104 38366 38552 + cds coding_transcript
5462105 5463818 38553 40266 + intron coding_transcript
5462105 5463818 38553 40266 + intron coding_transcript
5463819 5464269 40267 40717 + exon coding_transcript
5463819 5464269 40267 40717 + cds coding_transcript
5463819 5464269 40267 40717 + exon coding_transcript
5463819 5464269 40267 40717 + cds coding_transcript
5464270 5465219 40718 41667 + intron coding_transcript
5464270 5465219 40718 41667 + intron coding_transcript
5465220 5465505 41668 41953 + exon coding_transcript
5465220 5465505 41668 41953 + cds coding_transcript
5465220 5465505 41668 41953 + exon coding_transcript
5465220 5465505 41668 41953 + cds coding_transcript
5465506 5465893 41954 42341 + intron coding_transcript
5465506 5465893 41954 42341 + intron coding_transcript
5465894 5467610 42342 45120 + exon coding_transcript
5465894 5466829 42342 44339 + exon coding_transcript
5465894 5466712 42342 44222 + cds coding_transcript
5465894 5466712 42342 44222 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 43158 43217 + cds 2xTY1 CLC
1548 2264 43224 43940 + cds SGFP CLC
2271 2312 43947 43988 + cds V5 CLC
2313 2336 43989 44012 + cds Precision cut site CLC
2337 2357 44013 44033 + cds TEV CLC
2358 2429 44034 44105 + cds BLRP CLC
3813 3846 44113 44146 + misc_recomb FRT CLC
dmel-5.43-2L
5466713 5467610 44223 45120 + three_prime_utr coding_transcript
5466713 5466829 44223 44339 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
mid[43157]::S000169_fly_pretag_fwd
CTGCCGCCGGTTCGCCACCCGCGGTAAACGCCTCACGCAGCCCCGCCCACgaagtgcataccaatcaggacccgc
fasta Fasta
mid[43157]::S000169_fly_pretag_rev
TTTTTAATACTGCTCGTGGCAAGAACTTGGTGGCGTGGGCTGTTAACTTActtgtcgtcgtcatccttgtagtca
fasta Fasta