Clone: 14763769501329638 B06

Tagged gene: ckd
Identifier: FBgn0035427

Clone FlyFos015691(pRedFlp-Hgr)(ckd[19385]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
3271038 3276255 10805 16022 - gene CG14960 coding_transcript
3268495 3268994 18066 18565 - gene CG34265 coding_transcript
3258155 3267845 19215 29967 - gene ckd coding_transcript
3258155 3267845 19215 29967 - mrna FBtr0073050 coding_transcript
3267609 3267845 19215 20513 - exon coding_transcript
3267678 3267845 19215 19382 - three_prime_utr coding_transcript
3267609 3267677 19383 20513 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19459 19492 - misc_recomb FRT CLC
2358 2429 19500 19571 - cds BLRP CLC
2337 2357 19572 19592 - cds TEV CLC
2313 2336 19593 19616 - cds Precision cut site CLC
2271 2312 19617 19658 - cds V5 CLC
1548 2264 19665 20381 - cds SGFP CLC
1482 1541 20388 20447 - cds 2xTY1 CLC
dmel-5.43-3L
3267548 3267608 20514 20574 - intron coding_transcript
3267425 3267547 20575 20697 - exon coding_transcript
3267425 3267547 20575 20697 - cds coding_transcript
3267288 3267424 20698 20834 - intron coding_transcript
3267121 3267287 20835 21001 - exon coding_transcript
3267121 3267287 20835 21001 - cds coding_transcript
3266715 3267120 21002 21407 - intron coding_transcript
3266403 3266714 21408 21719 - exon coding_transcript
3266651 3266714 21408 21471 - cds coding_transcript
3266403 3266650 21472 21719 - five_prime_utr coding_transcript
3258656 3266402 21720 29466 - intron coding_transcript
3258155 3258655 29467 29967 - exon coding_transcript
3258155 3258655 29467 29967 - five_prime_utr coding_transcript
3248015 3249045 39077 40107 - gene CG43389 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
ckd[19385]::S000169_fly_pretag_rev
TTTGCTTTTGGAGACACACAATAAATATTTAATTAAGTGCAAATCGTTCActtgtcgtcgtcatccttgtagtca
fasta Fasta
ckd[19385]::S000169_fly_pretag_fwd
ACGAACTGACCTATGCCACAGATCCGCCAGATGAAGATGACGAGGATTACgaagtgcataccaatcaggacccgc
fasta Fasta