Clone: 14763769501329638 F05

Tagged gene: CG31459
Identifier: FBgn0051459

Clone FlyFos015686(pRedFlp-Hgr)(CG31459[19575]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
15666567 15667767 19446 21708 - gene CG31459 coding_transcript
15666567 15667767 19446 21708 - mrna FBtr0083812 coding_transcript
15667449 15667767 19446 20826 - exon coding_transcript
15667641 15667767 19446 19572 - three_prime_utr coding_transcript
15667449 15667640 19573 20826 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19649 19682 - misc_recomb FRT CLC
2358 2429 19690 19761 - cds BLRP CLC
2337 2357 19762 19782 - cds TEV CLC
2313 2336 19783 19806 - cds Precision cut site CLC
2271 2312 19807 19848 - cds V5 CLC
1548 2264 19855 20571 - cds SGFP CLC
1482 1541 20578 20637 - cds 2xTY1 CLC
dmel-5.43-3R
15667389 15667448 20827 20886 - intron coding_transcript
15667275 15667388 20887 21000 - exon coding_transcript
15667275 15667388 20887 21000 - cds coding_transcript
15667211 15667274 21001 21064 - intron coding_transcript
15666850 15667210 21065 21425 - exon coding_transcript
15666850 15667210 21065 21425 - cds coding_transcript
15666792 15666849 21426 21483 - intron coding_transcript
15666567 15666791 21484 21708 - exon coding_transcript
15666718 15666791 21484 21557 - cds coding_transcript
15666567 15666717 21558 21708 - five_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG31459[19575]::S000169_fly_pretag_rev
AAAGTTTATGTTACATATTTATGCATCACTTTTTTTTTGAGGATTTCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG31459[19575]::S000169_fly_pretag_fwd
GAGCCGCCGAATCCGGAATGTATATGGGCGATCAATTGGATTACAATAACgaagtgcataccaatcaggacccgc
fasta Fasta