Clone: 14763769501329638 A03

Tagged gene: Wsck
Identifier: FBgn0046685

Clone FlyFos015663(pRedFlp-Hgr)(Wsck[21425]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
20449524 20451302 17849 19627 - gene Syx18 coding_transcript
20451397 20460436 19722 29823 + gene atl coding_transcript
20452796 20455810 21121 25197 - gene Wsck coding_transcript
20452796 20455810 21121 25197 - mrna FBtr0084669 coding_transcript
20452796 20454384 21121 23771 - exon coding_transcript
20452796 20453097 21121 21422 - three_prime_utr coding_transcript
20453098 20454384 21423 23771 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 21499 21532 - misc_recomb FRT CLC
2358 2429 21540 21611 - cds BLRP CLC
2337 2357 21612 21632 - cds TEV CLC
2313 2336 21633 21656 - cds Precision cut site CLC
2271 2312 21657 21698 - cds V5 CLC
1548 2264 21705 22421 - cds SGFP CLC
1482 1541 22428 22487 - cds 2xTY1 CLC
dmel-5.43-3R
20454385 20454437 23772 23824 - intron coding_transcript
20454438 20454794 23825 24181 - exon coding_transcript
20454438 20454794 23825 24181 - cds coding_transcript
20454795 20454856 24182 24243 - intron coding_transcript
20454857 20455810 24244 25197 - exon coding_transcript
20454857 20455588 24244 24975 - cds coding_transcript
20455589 20455810 24976 25197 - five_prime_utr coding_transcript
20460426 20460887 29813 30274 - gene CG43222 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Wsck[21425]::S000169_fly_pretag_rev
ATGTGGTCTCTCGCTGACTAAAGCTAATTATACTATTCGGCTGCACCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta
Wsck[21425]::S000169_fly_pretag_fwd
ACACCCTGCCTCCGTACCTGCCGCAACTGGAAGCCGTTGCTACGATGGGCgaagtgcataccaatcaggacccgc
fasta Fasta