Clone: 3185971607969933 D12
Tagged gene: CG10097
Identifier: FBgn0038033
Clone | FlyFos015516(pRedFlp-Hgr)(CG10097[38541]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
---|---|
Library | FlyFos 5.43 D.mel in E. coli EPI300 |
Genome | Species: Drosophila melanogaster, Release: 5.43 |
Validation(click to hide details)
Type | Status | Download / View |
---|---|---|
Grows in selection | good | |
NGS-based tag validation | bad | |
Whole tag sequencing | flipped | download [show/hide details] |
GENOME<<>>TAG TAG<<>>GENOME PREDICTED TTGCAGGTTTTATGTATTGGACAGGATTCTTAAAACTGTATTAGTTACATTATTTGGTGGCTTTATTTGGTTATTAATCACAAATTTAGTGCCACTACTGGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAACTATTTTTTTTTATCTCTTAAATGAAATTATCTCCAGAAAATAACTGTAAGCCACATTGGTTTTTACAGGTTTTATGTATTGGACAGGATTCTTAAA COVERAGE 11111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111222212222212222122222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111122222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| EV6 TTGCAGGTTTTATGTATTGGACAGGATTCTTAAAACTGTATTAGTTACATTATTTGGTGGCTTTATTTGGTTATTAATCACAAATTTAGTGCCACTACTGGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCC |||| ||||| |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| DS2 TGGA-GGCGA-GTGA-CGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGT |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| EV1 TGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGT |
Genes in pFlyFos vector(click to hide details)
Gene | Clone relative | Genome relative | |||||
---|---|---|---|---|---|---|---|
Name | Length | Start | End | Strand | Start | End | Strand |
DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
---|---|---|---|---|---|---|---|---|
pFlyFos | ||||||||
1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
dmel-5.43-3R | ||||||||
8191902 | 8193286 | 10604 | 11988 | - | gene | GstD9 | coding_transcript | |
8193269 | 8194987 | 11971 | 13689 | - | gene | GstD1 | coding_transcript | |
8197693 | 8198426 | 16395 | 17128 | + | gene | GstD2 | coding_transcript | |
8198790 | 8199535 | 17492 | 18237 | + | gene | GstD3 | coding_transcript | |
8199824 | 8200546 | 18526 | 19248 | + | gene | GstD4 | coding_transcript | |
8201486 | 8202136 | 20188 | 20838 | + | gene | GstD5 | coding_transcript | |
8202894 | 8203629 | 21596 | 22331 | + | gene | GstD6 | coding_transcript | |
8204261 | 8204977 | 22963 | 23679 | + | gene | GstD7 | coding_transcript | |
8205745 | 8206537 | 24447 | 25239 | + | gene | GstD8 | coding_transcript | |
8207373 | 8208412 | 26075 | 27114 | - | gene | CG10035 | coding_transcript | |
8209083 | 8211348 | 27785 | 30050 | + | gene | CG17639 | coding_transcript | |
8213287 | 8214519 | 31989 | 33221 | + | gene | CG33098 | coding_transcript | |
8216405 | 8221702 | 35107 | 41466 | - | gene | CG10096 | coding_transcript | |
8216405 | 8221702 | 35107 | 41466 | - | mrna | FBtr0082603 | coding_transcript | |
8216405 | 8221702 | 35107 | 41466 | - | gene | CG10097 | coding_transcript | |
8216405 | 8221702 | 35107 | 41466 | - | mrna | FBtr0100524 | coding_transcript | |
8216405 | 8218733 | 35107 | 37435 | - | mrna | FBtr0082604 | coding_transcript | |
8216405 | 8216727 | 35107 | 35429 | - | exon | coding_transcript | ||
8216405 | 8216727 | 35107 | 35429 | - | exon | coding_transcript | ||
8216405 | 8216727 | 35107 | 35429 | - | exon | coding_transcript | ||
8216405 | 8216727 | 35107 | 35429 | - | three_prime_utr | coding_transcript | ||
8216405 | 8216627 | 35107 | 35329 | - | three_prime_utr | coding_transcript | ||
8216405 | 8216627 | 35107 | 35329 | - | three_prime_utr | coding_transcript | ||
8216628 | 8216727 | 35330 | 35429 | - | cds | coding_transcript | ||
8216628 | 8216727 | 35330 | 35429 | - | cds | coding_transcript | ||
8216728 | 8216805 | 35430 | 35507 | - | intron | coding_transcript | ||
8216728 | 8216805 | 35430 | 35507 | - | intron | coding_transcript | ||
8216728 | 8216805 | 35430 | 35507 | - | intron | coding_transcript | ||
8216806 | 8217514 | 35508 | 36216 | - | exon | coding_transcript | ||
8216806 | 8217514 | 35508 | 36216 | - | cds | coding_transcript | ||
8216806 | 8217514 | 35508 | 36216 | - | exon | coding_transcript | ||
8216806 | 8217514 | 35508 | 36216 | - | cds | coding_transcript | ||
8216806 | 8217514 | 35508 | 36216 | - | exon | coding_transcript | ||
8216806 | 8217514 | 35508 | 36216 | - | three_prime_utr | coding_transcript | ||
8217515 | 8217569 | 36217 | 36271 | - | intron | coding_transcript | ||
8217515 | 8217569 | 36217 | 36271 | - | intron | coding_transcript | ||
8217515 | 8217569 | 36217 | 36271 | - | intron | coding_transcript | ||
8217570 | 8218080 | 36272 | 36782 | - | exon | coding_transcript | ||
8217570 | 8218080 | 36272 | 36782 | - | cds | coding_transcript | ||
8217570 | 8218080 | 36272 | 36782 | - | exon | coding_transcript | ||
8217570 | 8218080 | 36272 | 36782 | - | cds | coding_transcript | ||
8217570 | 8218080 | 36272 | 36782 | - | exon | coding_transcript | ||
8217570 | 8218080 | 36272 | 36782 | - | three_prime_utr | coding_transcript | ||
8218081 | 8218180 | 36783 | 36882 | - | intron | coding_transcript | ||
8218081 | 8218180 | 36783 | 36882 | - | intron | coding_transcript | ||
8218081 | 8218180 | 36783 | 36882 | - | intron | coding_transcript | ||
8218181 | 8219933 | 36883 | 39697 | - | exon | coding_transcript | ||
8218181 | 8219933 | 36883 | 39697 | - | exon | coding_transcript | ||
8218181 | 8219836 | 36883 | 38538 | - | three_prime_utr | coding_transcript | ||
8218181 | 8218293 | 36883 | 36995 | - | exon | coding_transcript | ||
8218181 | 8218293 | 36883 | 36995 | - | cds | coding_transcript | ||
8218181 | 8218216 | 36883 | 36918 | - | cds | coding_transcript | ||
8218217 | 8219933 | 36919 | 39697 | - | five_prime_utr | coding_transcript | ||
8218294 | 8218548 | 36996 | 37250 | - | intron | coding_transcript | ||
8218549 | 8218733 | 37251 | 37435 | - | exon | coding_transcript | ||
8218549 | 8218624 | 37251 | 37326 | - | cds | coding_transcript | ||
8218625 | 8218733 | 37327 | 37435 | - | five_prime_utr | coding_transcript | ||
8219837 | 8221702 | 38539 | 41466 | - | mrna | FBtr0100525 | coding_transcript | |
8219837 | 8219933 | 38539 | 39697 | - | cds | coding_transcript | ||
8219837 | 8219933 | 38539 | 39697 | - | exon | coding_transcript | ||
8219837 | 8219933 | 38539 | 39697 | - | cds | coding_transcript | ||
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
2437 | 2470 | 38615 | 38648 | - | misc_recomb | FRT | CLC | |
2358 | 2429 | 38656 | 38727 | - | cds | BLRP | CLC | |
2337 | 2357 | 38728 | 38748 | - | cds | TEV | CLC | |
2313 | 2336 | 38749 | 38772 | - | cds | Precision cut site | CLC | |
2271 | 2312 | 38773 | 38814 | - | cds | V5 | CLC | |
1548 | 2264 | 38821 | 39537 | - | cds | SGFP | CLC | |
1482 | 1541 | 39544 | 39603 | - | cds | 2xTY1 | CLC | |
dmel-5.43-3R | ||||||||
8219934 | 8219992 | 39698 | 39756 | - | intron | coding_transcript | ||
8219934 | 8219992 | 39698 | 39756 | - | intron | coding_transcript | ||
8219934 | 8219992 | 39698 | 39756 | - | intron | coding_transcript | ||
8219993 | 8221545 | 39757 | 41309 | - | exon | coding_transcript | ||
8219993 | 8221545 | 39757 | 41309 | - | five_prime_utr | coding_transcript | ||
8219993 | 8220701 | 39757 | 40465 | - | exon | coding_transcript | ||
8219993 | 8220701 | 39757 | 40465 | - | cds | coding_transcript | ||
8219993 | 8220701 | 39757 | 40465 | - | exon | coding_transcript | ||
8219993 | 8220701 | 39757 | 40465 | - | cds | coding_transcript | ||
8220702 | 8220797 | 40466 | 40561 | - | intron | coding_transcript | ||
8220702 | 8220797 | 40466 | 40561 | - | intron | coding_transcript | ||
8220798 | 8221545 | 40562 | 41309 | - | exon | coding_transcript | ||
8220798 | 8221341 | 40562 | 41105 | - | cds | coding_transcript | ||
8220798 | 8221308 | 40562 | 41072 | - | exon | coding_transcript | ||
8220798 | 8221308 | 40562 | 41072 | - | cds | coding_transcript | ||
8221309 | 8221432 | 41073 | 41196 | - | intron | coding_transcript | ||
8221342 | 8221545 | 41106 | 41309 | - | five_prime_utr | coding_transcript | ||
8221433 | 8221545 | 41197 | 41309 | - | exon | coding_transcript | ||
8221433 | 8221545 | 41197 | 41309 | - | cds | coding_transcript | ||
8221546 | 8221615 | 41310 | 41379 | - | intron | coding_transcript | ||
8221546 | 8221615 | 41310 | 41379 | - | intron | coding_transcript | ||
8221546 | 8221615 | 41310 | 41379 | - | intron | coding_transcript | ||
8221616 | 8221702 | 41380 | 41466 | - | exon | coding_transcript | ||
8221616 | 8221702 | 41380 | 41466 | - | five_prime_utr | coding_transcript | ||
8221616 | 8221702 | 41380 | 41466 | - | exon | coding_transcript | ||
8221616 | 8221702 | 41380 | 41466 | - | five_prime_utr | coding_transcript | ||
8221616 | 8221702 | 41380 | 41466 | - | exon | coding_transcript | ||
8221616 | 8221691 | 41380 | 41455 | - | cds | coding_transcript | ||
8221692 | 8221702 | 41456 | 41466 | - | five_prime_utr | coding_transcript | ||
8224733 | 8227535 | 44497 | 47299 | - | gene | lig3 | coding_transcript |
Sets(click to hide details)
Oligos(click to hide details)
Name/Sequence | Export |
---|---|
CG10097[38541]::S000169_fly_pretag_fwd
TATTTGGTGGCTTTATTTGGTTATTAATCACAAATTTAGTGCCACTACTGgaagtgcataccaatcaggacccgc
|
Fasta |
CG10097[38541]::S000169_fly_pretag_rev
CAGTTATTTTCTGGAGATAATTTCATTTAAGAGATAAAAAAAAATAGTTActtgtcgtcgtcatccttgtagtca
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Fasta |