Clone: 3185971607969933 B10
Tagged gene: CG17724
Identifier: FBgn0033802
| Clone | FlyFos015482(pRedFlp-Hgr)(CG17724[17580]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad | |
| Whole tag sequencing | incomplete | download [show/hide details] |
GENOME<<>>TAG TAG<<>>GENOME
PREDICTED CACAAATCCCCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACAGCAACAGGCTGCCGCTGCTGCTGCTGCTCAGTTGTTCGCCGCGGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAGGGTCTAATGGGTGGCCAAATGGGTCGATTTTTGAACAGTGCCACCATAATGCCGACCACATACTTCTTTTAGAAATCTCAAAAATGTTCCGCGCAGA
COVERAGE 11111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222221111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111112222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222222022222222222222222222222222222222222222222222222222222222222222122222222222222222
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EV6 CACAAATCCCCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACAGCAACAGGCTGCCGCTGCTGCTGCTGCTCAGTTGTTCGCCGCGGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACC
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DS2 ACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAGGGTCTAATGGGTGGCC-AATGGGTCGATTTTTGAACAGTGCCACCATAATGCCGACCACATACTTCTTTTAGAAATCTC-AAAATGTTCCGCGCAGA
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EV1 GGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAGGGTCTAATGGGTGGCC-AATGGGTCGATTTTTGAACAGTGCCACCATAATGCCGACCACATACTTCTTTTAGAAATCTCAAAAATGTTCCGCGCAGA
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Genes in pFlyFos vector
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-2R | ||||||||
| 9058908 | 9062301 | 11556 | 14949 | + | gene | CG33138 | coding_transcript | |
| 9062941 | 9063580 | 15589 | 16228 | + | gene | CG33137 | coding_transcript | |
| 9063599 | 9085869 | 16247 | 39579 | - | gene | Kdm4B | coding_transcript | |
| 9063599 | 9073317 | 16247 | 27027 | - | gene | CG17724 | coding_transcript | |
| 9063599 | 9073317 | 16247 | 27027 | - | mrna | FBtr0087771 | coding_transcript | |
| 9063599 | 9072718 | 16247 | 26428 | - | mrna | FBtr0310499 | coding_transcript | |
| 9063599 | 9066518 | 16247 | 20228 | - | exon | coding_transcript | ||
| 9063599 | 9066518 | 16247 | 20228 | - | exon | coding_transcript | ||
| 9063599 | 9066518 | 16247 | 20228 | - | exon | coding_transcript | ||
| 9063599 | 9066518 | 16247 | 20228 | - | three_prime_utr | coding_transcript | ||
| 9063599 | 9064929 | 16247 | 17577 | - | three_prime_utr | coding_transcript | ||
| 9063599 | 9064929 | 16247 | 17577 | - | three_prime_utr | coding_transcript | ||
| 9064930 | 9066486 | 17578 | 20196 | - | cds | coding_transcript | ||
| 9064930 | 9066486 | 17578 | 20196 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 17654 | 17687 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 17695 | 17766 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 17767 | 17787 | - | cds | TEV | CLC | |
| 2313 | 2336 | 17788 | 17811 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 17812 | 17853 | - | cds | V5 | CLC | |
| 1548 | 2264 | 17860 | 18576 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 18583 | 18642 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-2R | ||||||||
| 9066487 | 9066518 | 20197 | 20228 | - | five_prime_utr | coding_transcript | ||
| 9066487 | 9066518 | 20197 | 20228 | - | five_prime_utr | coding_transcript | ||
| 9066519 | 9067092 | 20229 | 20802 | - | intron | coding_transcript | ||
| 9066519 | 9067092 | 20229 | 20802 | - | intron | coding_transcript | ||
| 9066519 | 9067092 | 20229 | 20802 | - | intron | coding_transcript | ||
| 9066673 | 9077579 | 20383 | 31289 | - | gene | seq | coding_transcript | |
| 9066673 | 9067615 | 20383 | 21325 | - | exon | coding_transcript | ||
| 9066673 | 9067578 | 20383 | 21288 | - | three_prime_utr | coding_transcript | ||
| 9067093 | 9067615 | 20803 | 21325 | - | exon | coding_transcript | ||
| 9067093 | 9067615 | 20803 | 21325 | - | five_prime_utr | coding_transcript | ||
| 9067093 | 9067615 | 20803 | 21325 | - | exon | coding_transcript | ||
| 9067093 | 9067615 | 20803 | 21325 | - | five_prime_utr | coding_transcript | ||
| 9067093 | 9067615 | 20803 | 21325 | - | exon | coding_transcript | ||
| 9067093 | 9067586 | 20803 | 21296 | - | three_prime_utr | coding_transcript | ||
| 9067579 | 9067615 | 21289 | 21325 | - | cds | coding_transcript | ||
| 9067587 | 9067615 | 21297 | 21325 | - | cds | coding_transcript | ||
| 9067616 | 9072281 | 21326 | 25991 | - | intron | coding_transcript | ||
| 9067616 | 9072281 | 21326 | 25991 | - | intron | coding_transcript | ||
| 9067616 | 9072281 | 21326 | 25991 | - | intron | coding_transcript | ||
| 9067616 | 9068494 | 21326 | 22204 | - | intron | coding_transcript | ||
| 9068495 | 9070739 | 22205 | 24449 | - | exon | coding_transcript | ||
| 9068495 | 9070739 | 22205 | 24449 | - | cds | coding_transcript | ||
| 9070740 | 9072281 | 24450 | 25991 | - | intron | coding_transcript | ||
| 9072282 | 9072718 | 25992 | 26428 | - | exon | coding_transcript | ||
| 9072282 | 9072718 | 25992 | 26428 | - | five_prime_utr | coding_transcript | ||
| 9072282 | 9072680 | 25992 | 26390 | - | exon | coding_transcript | ||
| 9072282 | 9072680 | 25992 | 26390 | - | five_prime_utr | coding_transcript | ||
| 9072282 | 9072680 | 25992 | 26390 | - | exon | coding_transcript | ||
| 9072282 | 9072680 | 25992 | 26390 | - | exon | coding_transcript | ||
| 9072282 | 9072680 | 25992 | 26390 | - | cds | coding_transcript | ||
| 9072282 | 9072648 | 25992 | 26358 | - | cds | coding_transcript | ||
| 9072649 | 9072680 | 26359 | 26390 | - | five_prime_utr | coding_transcript | ||
| 9072681 | 9073288 | 26391 | 26998 | - | intron | coding_transcript | ||
| 9072681 | 9073288 | 26391 | 26998 | - | intron | coding_transcript | ||
| 9073039 | 9073241 | 26749 | 26951 | - | three_prime_utr | coding_transcript | ||
| 9073039 | 9073241 | 26749 | 26951 | - | three_prime_utr | coding_transcript | ||
| 9073039 | 9073241 | 26749 | 26951 | - | three_prime_utr | coding_transcript | ||
| 9073039 | 9073241 | 26749 | 26951 | - | three_prime_utr | coding_transcript | ||
| 9073289 | 9073317 | 26999 | 27027 | - | exon | coding_transcript | ||
| 9073289 | 9073317 | 26999 | 27027 | - | five_prime_utr | coding_transcript | ||
| 9086356 | 9090217 | 40066 | 43927 | + | gene | Aats-val | coding_transcript | |
| 9090248 | 9094027 | 43958 | 47737 | - | gene | CG34315 | coding_transcript | |
| 9090248 | 9094027 | 43958 | 47737 | - | gene | lat | coding_transcript | |
Sets
Oligos
| Name/Sequence | Export |
|---|---|
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CG17724[17580]::S000169_fly_pretag_rev
ATGGTGGCACTGTTCAAAAATCGACCCATTTGGCCACCCATTAGACCCTActtgtcgtcgtcatccttgtagtca
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Fasta
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CG17724[17580]::S000169_fly_pretag_fwd
AACAACAGCAACAGGCTGCCGCTGCTGCTGCTGCTCAGTTGTTCGCCGCGgaagtgcataccaatcaggacccgc
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Fasta
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