Clone: 3185971607969933 B09
Tagged gene: CG11768
Identifier: FBgn0037625
| Clone | FlyFos015476(pRedFlp-Hgr)(CG11768[18117]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad | |
| Whole tag sequencing | flipped | download [show/hide details] |
GENOME<<>>TAG TAG<<>>GENOME
PREDICTED GCAGCACAGTGAGTCACAGGTCACTCTGCAGCCGCATCTGAGTACGTATTCAGGGGCAGCCGGTTTGTTAATCCCAGTCCCAGATCCACATCCGCTCTACGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAACTTTCTGGGGGAAATCTCCCGTATTTATTGTGTACACTTTTTGCCTTCCTACTTAGTTTTTCTTTTAATACAAAACATTATTTTATGTACGCACTTA
COVERAGE 00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111222222222222222222222222222222222222111111111111111111111111111111111111111111111111111111111111111111111111110000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
EV5 TGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGT
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DS88 GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAA
|
||
Genes in pFlyFos vector
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-3R | ||||||||
| 4573528 | 4581638 | 11812 | 20984 | + | gene | CG8223 | coding_transcript | |
| 4574927 | 4593922 | 13211 | 33268 | - | gene | CG11768 | coding_transcript | |
| 4574927 | 4593913 | 13211 | 33259 | - | mrna | FBtr0308890 | coding_transcript | |
| 4574927 | 4576435 | 13211 | 14719 | - | exon | coding_transcript | ||
| 4574927 | 4576435 | 13211 | 14719 | - | three_prime_utr | coding_transcript | ||
| 4574928 | 4593913 | 13212 | 33259 | - | gene | CG34135 | coding_transcript | |
| 4574928 | 4593913 | 13212 | 33259 | - | mrna | FBtr0308891 | coding_transcript | |
| 4574928 | 4578463 | 13212 | 16747 | - | mrna | FBtr0110905 | coding_transcript | |
| 4574928 | 4576435 | 13212 | 14719 | - | exon | coding_transcript | ||
| 4574928 | 4576435 | 13212 | 14719 | - | exon | coding_transcript | ||
| 4574928 | 4576154 | 13212 | 14438 | - | three_prime_utr | coding_transcript | ||
| 4574928 | 4576154 | 13212 | 14438 | - | three_prime_utr | coding_transcript | ||
| 4576155 | 4576435 | 14439 | 14719 | - | cds | coding_transcript | ||
| 4576155 | 4576435 | 14439 | 14719 | - | cds | coding_transcript | ||
| 4576436 | 4576501 | 14720 | 14785 | - | intron | coding_transcript | ||
| 4576436 | 4576501 | 14720 | 14785 | - | intron | coding_transcript | ||
| 4576436 | 4576501 | 14720 | 14785 | - | intron | coding_transcript | ||
| 4576502 | 4580132 | 14786 | 19478 | - | exon | coding_transcript | ||
| 4576502 | 4580132 | 14786 | 19478 | - | exon | coding_transcript | ||
| 4576502 | 4579830 | 14786 | 18114 | - | three_prime_utr | coding_transcript | ||
| 4576502 | 4578463 | 14786 | 16747 | - | exon | coding_transcript | ||
| 4576502 | 4576637 | 14786 | 14921 | - | cds | coding_transcript | ||
| 4576502 | 4576637 | 14786 | 14921 | - | cds | coding_transcript | ||
| 4576638 | 4580132 | 14922 | 19478 | - | five_prime_utr | coding_transcript | ||
| 4576638 | 4578463 | 14922 | 16747 | - | five_prime_utr | coding_transcript | ||
| 4577783 | 4578120 | 16067 | 16404 | + | exon | coding_transcript | ||
| 4577783 | 4578120 | 16067 | 16404 | + | exon | coding_transcript | ||
| 4577783 | 4577803 | 16067 | 16087 | + | five_prime_utr | coding_transcript | ||
| 4577783 | 4577803 | 16067 | 16087 | + | five_prime_utr | coding_transcript | ||
| 4577804 | 4578120 | 16088 | 16404 | + | cds | coding_transcript | ||
| 4577804 | 4578120 | 16088 | 16404 | + | cds | coding_transcript | ||
| 4578121 | 4578235 | 16405 | 16519 | + | intron | coding_transcript | ||
| 4578121 | 4578235 | 16405 | 16519 | + | intron | coding_transcript | ||
| 4578236 | 4578840 | 16520 | 17124 | + | exon | coding_transcript | ||
| 4578236 | 4578840 | 16520 | 17124 | + | cds | coding_transcript | ||
| 4578236 | 4578840 | 16520 | 17124 | + | exon | coding_transcript | ||
| 4578236 | 4578840 | 16520 | 17124 | + | cds | coding_transcript | ||
| 4578841 | 4578902 | 17125 | 17186 | + | intron | coding_transcript | ||
| 4578841 | 4578902 | 17125 | 17186 | + | intron | coding_transcript | ||
| 4578903 | 4579180 | 17187 | 17464 | + | exon | coding_transcript | ||
| 4578903 | 4579180 | 17187 | 17464 | + | cds | coding_transcript | ||
| 4578903 | 4579180 | 17187 | 17464 | + | exon | coding_transcript | ||
| 4578903 | 4579180 | 17187 | 17464 | + | cds | coding_transcript | ||
| 4579181 | 4579242 | 17465 | 17526 | + | intron | coding_transcript | ||
| 4579181 | 4579242 | 17465 | 17526 | + | intron | coding_transcript | ||
| 4579243 | 4581638 | 17527 | 20984 | + | exon | coding_transcript | ||
| 4579243 | 4579827 | 17527 | 18111 | + | exon | coding_transcript | ||
| 4579243 | 4579521 | 17527 | 17805 | + | cds | coding_transcript | ||
| 4579243 | 4579521 | 17527 | 17805 | + | cds | coding_transcript | ||
| 4579522 | 4581638 | 17806 | 20984 | + | three_prime_utr | coding_transcript | ||
| 4579522 | 4579827 | 17806 | 18111 | + | three_prime_utr | coding_transcript | ||
| 4579760 | 4593922 | 18044 | 33268 | - | mrna | FBtr0302489 | coding_transcript | |
| 4579760 | 4593913 | 18044 | 33259 | - | mrna | FBtr0081943 | coding_transcript | |
| 4579760 | 4580132 | 18044 | 19478 | - | exon | coding_transcript | ||
| 4579760 | 4580132 | 18044 | 19478 | - | exon | coding_transcript | ||
| 4579760 | 4579830 | 18044 | 18114 | - | three_prime_utr | coding_transcript | ||
| 4579760 | 4579830 | 18044 | 18114 | - | three_prime_utr | coding_transcript | ||
| 4579831 | 4580132 | 18115 | 19478 | - | cds | coding_transcript | ||
| 4579831 | 4580132 | 18115 | 19478 | - | cds | coding_transcript | ||
| 4579831 | 4580132 | 18115 | 19478 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 18191 | 18224 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 18232 | 18303 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 18304 | 18324 | - | cds | TEV | CLC | |
| 2313 | 2336 | 18325 | 18348 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 18349 | 18390 | - | cds | V5 | CLC | |
| 1548 | 2264 | 18397 | 19113 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 19120 | 19179 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-3R | ||||||||
| 4580133 | 4580208 | 19479 | 19554 | - | intron | coding_transcript | ||
| 4580133 | 4580208 | 19479 | 19554 | - | intron | coding_transcript | ||
| 4580133 | 4580208 | 19479 | 19554 | - | intron | coding_transcript | ||
| 4580133 | 4580208 | 19479 | 19554 | - | intron | coding_transcript | ||
| 4580209 | 4581934 | 19555 | 21280 | - | exon | coding_transcript | ||
| 4580209 | 4581934 | 19555 | 21280 | - | exon | coding_transcript | ||
| 4580209 | 4581934 | 19555 | 21280 | - | exon | coding_transcript | ||
| 4580209 | 4581934 | 19555 | 21280 | - | exon | coding_transcript | ||
| 4580209 | 4581934 | 19555 | 21280 | - | five_prime_utr | coding_transcript | ||
| 4580209 | 4581391 | 19555 | 20737 | - | cds | coding_transcript | ||
| 4580209 | 4581391 | 19555 | 20737 | - | cds | coding_transcript | ||
| 4580209 | 4581391 | 19555 | 20737 | - | cds | coding_transcript | ||
| 4581392 | 4581934 | 20738 | 21280 | - | five_prime_utr | coding_transcript | ||
| 4581392 | 4581934 | 20738 | 21280 | - | five_prime_utr | coding_transcript | ||
| 4581392 | 4581934 | 20738 | 21280 | - | five_prime_utr | coding_transcript | ||
| 4581935 | 4593055 | 21281 | 32401 | - | intron | coding_transcript | ||
| 4581935 | 4593055 | 21281 | 32401 | - | intron | coding_transcript | ||
| 4581935 | 4593055 | 21281 | 32401 | - | intron | coding_transcript | ||
| 4581935 | 4593055 | 21281 | 32401 | - | intron | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | exon | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | five_prime_utr | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | exon | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | five_prime_utr | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | exon | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | five_prime_utr | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | exon | coding_transcript | ||
| 4593056 | 4593516 | 32402 | 32862 | - | five_prime_utr | coding_transcript | ||
| 4593517 | 4593841 | 32863 | 33187 | - | intron | coding_transcript | ||
| 4593517 | 4593841 | 32863 | 33187 | - | intron | coding_transcript | ||
| 4593517 | 4593841 | 32863 | 33187 | - | intron | coding_transcript | ||
| 4593517 | 4593796 | 32863 | 33142 | - | intron | coding_transcript | ||
| 4593797 | 4593922 | 33143 | 33268 | - | exon | coding_transcript | ||
| 4593797 | 4593922 | 33143 | 33268 | - | five_prime_utr | coding_transcript | ||
| 4593842 | 4593913 | 33188 | 33259 | - | exon | coding_transcript | ||
| 4593842 | 4593913 | 33188 | 33259 | - | five_prime_utr | coding_transcript | ||
| 4593842 | 4593913 | 33188 | 33259 | - | exon | coding_transcript | ||
| 4593842 | 4593913 | 33188 | 33259 | - | five_prime_utr | coding_transcript | ||
| 4593842 | 4593913 | 33188 | 33259 | - | exon | coding_transcript | ||
| 4593842 | 4593913 | 33188 | 33259 | - | five_prime_utr | coding_transcript | ||
| 4595116 | 4596872 | 34462 | 36218 | + | gene | CG8236 | coding_transcript | |
| 4596968 | 4598408 | 36314 | 37754 | - | gene | CG13318 | coding_transcript | |
| 4599157 | 4599532 | 38503 | 38878 | + | gene | CG34301 | coding_transcript | |
| 4604101 | 4605645 | 43447 | 44991 | - | gene | CG33191 | coding_transcript | |
Sets
Oligos
| Name/Sequence | Export |
|---|---|
|
CG11768[18117]::S000169_fly_pretag_rev
AAGGCAAAAAGTGTACACAATAAATACGGGAGATTTCCCCCAGAAAGTTActtgtcgtcgtcatccttgtagtca
|
Fasta
|
|
CG11768[18117]::S000169_fly_pretag_fwd
CAGGGGCAGCCGGTTTGTTAATCCCAGTCCCAGATCCACATCCGCTCTACgaagtgcataccaatcaggacccgc
|
Fasta
|