Clone: 3185971607969933 B06
Tagged gene: CG10804
Identifier: FBgn0029663
| Clone | FlyFos015438(pRedFlp-Hgr)(CG10804[29298]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad | |
| Whole tag sequencing | flipped | download [show/hide details] |
GENOME<<>>TAG TAG<<>>GENOME
PREDICTED CGAACTGGAGCGCAGCATTTTCTGCTTCAACATGGACGGTACGGAGGGCATGTGTTGCCCAAAGTACGGACTACCCGAAAAGTCGCTGGAAGAGGAGGAGGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAGACACGCACAGGATACCAATGATAAGTTGACAACAACGACAATAAAACCAAATGAATTCTTGACCGAACCCAAACCGAATATGATACGAATGATGAAA
COVERAGE 00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
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EV5 TGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGT
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Genes in pFlyFos vector
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-X | ||||||||
| 3353049 | 3354273 | 9961 | 11185 | - | gene | CG16782 | coding_transcript | |
| 3354661 | 3356533 | 11573 | 13445 | + | gene | CG10801 | coding_transcript | |
| 3356503 | 3358077 | 13415 | 14989 | - | gene | CG16781 | coding_transcript | |
| 3359770 | 3368553 | 16682 | 25465 | - | gene | CG12206 | coding_transcript | |
| 3370709 | 3376355 | 27621 | 34329 | - | gene | CG10804 | coding_transcript | |
| 3370709 | 3376355 | 27621 | 34329 | - | mrna | FBtr0070591 | coding_transcript | |
| 3370709 | 3376355 | 27621 | 34329 | - | mrna | FBtr0070592 | coding_transcript | |
| 3370709 | 3374981 | 27621 | 32955 | - | mrna | FBtr0114487 | coding_transcript | |
| 3370709 | 3372582 | 27621 | 30556 | - | exon | coding_transcript | ||
| 3370709 | 3372582 | 27621 | 30556 | - | exon | coding_transcript | ||
| 3370709 | 3372582 | 27621 | 30556 | - | exon | coding_transcript | ||
| 3370709 | 3372383 | 27621 | 29295 | - | three_prime_utr | coding_transcript | ||
| 3370709 | 3372383 | 27621 | 29295 | - | three_prime_utr | coding_transcript | ||
| 3370709 | 3372383 | 27621 | 29295 | - | three_prime_utr | coding_transcript | ||
| 3372384 | 3372582 | 29296 | 30556 | - | cds | coding_transcript | ||
| 3372384 | 3372582 | 29296 | 30556 | - | cds | coding_transcript | ||
| 3372384 | 3372582 | 29296 | 30556 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 29372 | 29405 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 29413 | 29484 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 29485 | 29505 | - | cds | TEV | CLC | |
| 2313 | 2336 | 29506 | 29529 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 29530 | 29571 | - | cds | V5 | CLC | |
| 1548 | 2264 | 29578 | 30294 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 30301 | 30360 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-X | ||||||||
| 3372583 | 3372643 | 30557 | 30617 | - | intron | coding_transcript | ||
| 3372583 | 3372643 | 30557 | 30617 | - | intron | coding_transcript | ||
| 3372583 | 3372643 | 30557 | 30617 | - | intron | coding_transcript | ||
| 3372644 | 3373065 | 30618 | 31039 | - | exon | coding_transcript | ||
| 3372644 | 3373065 | 30618 | 31039 | - | cds | coding_transcript | ||
| 3372644 | 3373065 | 30618 | 31039 | - | exon | coding_transcript | ||
| 3372644 | 3373065 | 30618 | 31039 | - | cds | coding_transcript | ||
| 3372644 | 3373065 | 30618 | 31039 | - | exon | coding_transcript | ||
| 3372644 | 3373065 | 30618 | 31039 | - | cds | coding_transcript | ||
| 3373066 | 3373160 | 31040 | 31134 | - | intron | coding_transcript | ||
| 3373066 | 3373160 | 31040 | 31134 | - | intron | coding_transcript | ||
| 3373066 | 3373160 | 31040 | 31134 | - | intron | coding_transcript | ||
| 3373161 | 3374382 | 31135 | 32356 | - | exon | coding_transcript | ||
| 3373161 | 3374382 | 31135 | 32356 | - | cds | coding_transcript | ||
| 3373161 | 3373364 | 31135 | 31338 | - | exon | coding_transcript | ||
| 3373161 | 3373364 | 31135 | 31338 | - | cds | coding_transcript | ||
| 3373161 | 3373364 | 31135 | 31338 | - | exon | coding_transcript | ||
| 3373161 | 3373364 | 31135 | 31338 | - | cds | coding_transcript | ||
| 3373365 | 3373424 | 31339 | 31398 | - | intron | coding_transcript | ||
| 3373365 | 3373424 | 31339 | 31398 | - | intron | coding_transcript | ||
| 3373425 | 3374382 | 31399 | 32356 | - | exon | coding_transcript | ||
| 3373425 | 3374382 | 31399 | 32356 | - | cds | coding_transcript | ||
| 3373425 | 3374382 | 31399 | 32356 | - | exon | coding_transcript | ||
| 3373425 | 3374382 | 31399 | 32356 | - | cds | coding_transcript | ||
| 3374383 | 3374454 | 32357 | 32428 | - | intron | coding_transcript | ||
| 3374383 | 3374454 | 32357 | 32428 | - | intron | coding_transcript | ||
| 3374383 | 3374454 | 32357 | 32428 | - | intron | coding_transcript | ||
| 3374455 | 3374762 | 32429 | 32736 | - | exon | coding_transcript | ||
| 3374455 | 3374762 | 32429 | 32736 | - | exon | coding_transcript | ||
| 3374455 | 3374762 | 32429 | 32736 | - | exon | coding_transcript | ||
| 3374455 | 3374762 | 32429 | 32736 | - | cds | coding_transcript | ||
| 3374455 | 3374660 | 32429 | 32634 | - | cds | coding_transcript | ||
| 3374455 | 3374660 | 32429 | 32634 | - | cds | coding_transcript | ||
| 3374661 | 3374762 | 32635 | 32736 | - | five_prime_utr | coding_transcript | ||
| 3374661 | 3374762 | 32635 | 32736 | - | five_prime_utr | coding_transcript | ||
| 3374763 | 3374891 | 32737 | 32865 | - | intron | coding_transcript | ||
| 3374763 | 3374891 | 32737 | 32865 | - | intron | coding_transcript | ||
| 3374763 | 3374891 | 32737 | 32865 | - | intron | coding_transcript | ||
| 3374892 | 3374981 | 32866 | 32955 | - | exon | coding_transcript | ||
| 3374892 | 3374981 | 32866 | 32955 | - | cds | coding_transcript | ||
| 3374892 | 3374968 | 32866 | 32942 | - | exon | coding_transcript | ||
| 3374892 | 3374968 | 32866 | 32942 | - | five_prime_utr | coding_transcript | ||
| 3374892 | 3374968 | 32866 | 32942 | - | exon | coding_transcript | ||
| 3374892 | 3374968 | 32866 | 32942 | - | five_prime_utr | coding_transcript | ||
| 3374969 | 3376058 | 32943 | 34032 | - | intron | coding_transcript | ||
| 3374969 | 3376058 | 32943 | 34032 | - | intron | coding_transcript | ||
| 3376059 | 3376355 | 34033 | 34329 | - | exon | coding_transcript | ||
| 3376059 | 3376355 | 34033 | 34329 | - | five_prime_utr | coding_transcript | ||
| 3376059 | 3376355 | 34033 | 34329 | - | exon | coding_transcript | ||
| 3376059 | 3376355 | 34033 | 34329 | - | five_prime_utr | coding_transcript | ||
| 3377002 | 3378593 | 34976 | 36567 | + | gene | CG10802 | coding_transcript | |
| 3378633 | 3379407 | 36607 | 37381 | - | gene | CG14270 | coding_transcript | |
| 3379686 | 3382941 | 37660 | 40915 | + | gene | CG10803 | coding_transcript | |
Sets
Oligos
| Name/Sequence | Export |
|---|---|
|
CG10804[29298]::S000169_fly_pretag_rev
GTTTTATTGTCGTTGTTGTCAACTTATCATTGGTATCCTGTGCGTGTCTActtgtcgtcgtcatccttgtagtca
|
Fasta
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CG10804[29298]::S000169_fly_pretag_fwd
TGTGTTGCCCAAAGTACGGACTACCCGAAAAGTCGCTGGAAGAGGAGGAGgaagtgcataccaatcaggacccgc
|
Fasta
|