Welcome to The TransgeneOme Project website!
Currently the project website is under construction. Check regularly for updates.
The goal of the TransgeneOme project is to provide a platform for systematic description of the in vivo protein localization and physical interactions under native regulatory control. To that end we have developed an efficient high throughput transgene-based platform for protein tagging with fluorescent/affinity epitopes. We use in vivo recombination based DNA engineering (recombineering) in E. coli to seamlessly insert a tag coding sequence into genomic fosmid clones, containing the gene of interest in its native genomic environment. Stable integration of these large constructs into the genome result in reliable, near endogenous levels and patterns of gene expression.
The method is applicable to most model systems. In collaboration with other groups we have successfully applied this approach in several model systems including tissue culture cells, mice, C. elegans, Drosophila and Zebrafish.
We are now working towards extending this approach to a genome wide scale with the goal of complete TransgeneOme coverage of all protein coding genes in the commonly used model systems.
What is a TransgeneOme?
TransgeneOme |trɑːnsˈjēˌnōm|
noun Biology
• a comprehensive set of transgenes that covers an entire genome.
DERIVATIVES
TransgenOmic | trɑːnsjēˈnämik;| adjective
ORIGIN: a wordplay blend of transgene and ome.